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Paper
The following short technical report has been accepted by BioTechniques and is available online:
Holleley CE and Geerts PG ( 2009 )
Multiplex Manager 1.0: a crossplatform computer program that plans and optimizes multiplex PCR
BioTechniques, Vol 46, No 7, pp 511-517
Supplementary Materials
The paper refers to the Marshfield Clinic screening sets:
http://research.marshfieldclinic.org/genetics/GeneticResearch/screeningsets.asp
You can download The Marshfield Data Set as a Multiplex Manager
input file for Set 12 here: MarshfieldSet12.xml.
View a sample report generated for the The Marshfield Data Set here: Set 12 Report
This file was generated using the Import CSV Data option in the Utilities menu.
Sample File used in the Screencast:
Sample Input File.xml.
Studies that have used Multiplex Manager
- A parentage study using microsatellite loci in a pilot project for aquaculture of the European anchovy Engraulis encrasicolus L.
Aquaculture 310 305-311 DOI:16/j.aquaculture.2010.10.025Yaisel J. Borrell, Jorge Alvarez, Gloria Blanco, Amalia Mártinez De Murguía, Deborah Lee, Carlos Fernández, Concha Martínez, Unai Cotano, Paula álvarez, José Antonio Sanchez Prado
- Assessment of parental contributions to fast- and slow-growing progenies in the sea bream Sparus aurata L. using a new multiplex PCR
Aquaculture 314 58-65 DOI:16/j.aquaculture.2011.01.028Yaisel J. Borrell, Victor Gallego, Carmen García-Fernández, Ilaria Mazzeo, Luz Pérez, Juan F. Asturiano, Carlos E. Carleos, Emilia Vázquez, Jose A. Sánchez, Gloria Blanco
- Building genetic networks using relatedness information: a novel approach for the estimation of dispersal and characterization of group structure in social animals
Molecular Ecology DOI:10.1111/j.1365-294X.2012.05492.xLee Ann Rollins, Lucy E Browning, Clare E Holleley, James L Savage, Andrew F Russell, Simon C Griffith
- Comparison of random and SSR‐enriched shotgun pyrosequencing for microsatellite discovery and single multiplex PCR optimization in Acacia harpophylla
Molecular Ecology Resources 11 711-724 DOI:10.1111/j.1755-0998.2011.03002.xOlivier Lepais, Cecile F. E Bacles
- Cross-species amplification and development of microsatellites for six species of European Coenagrionid damselflies
Conservation Genetics Resources DOI:10.1007/s12686-011-9506-4Helena Johansson, Pär K. Ingvarsson, Frank Johansson
- Current trends in microsatellite genotyping
Molecular Ecology Resources 11 591-611 DOI:10.1111/j.1755-0998.2011.03014.xE. Guichoux, L. Lagache, S. Wagner, P. Chaumeil, P. Léger, O. Lepais, C. Lepoittevin, T. Malausa, E. Revardel, F. Salin, R. J Petit
- De Novo Discovery and Multiplexed Amplification of Microsatellite Markers for Black Alder (Alnus glutinosa) and Related Species Using SSR-Enriched Shotgun Pyrosequencing
Journal of Heredity DOI:10.1093/jhered/esr062Olivier Lepais, Cecile Fanny Emilie Bacles
- Development and characterization of 24 polymorphic microsatellite loci in two Antirrhinum maju subspecies (Plantaginaceae) using pyrosequencing technology
Conservation Genetics Resources DOI:10.1007/s12686-011-9478-4Gabriel D. G. Debout, Emeline Lhuillier, Pierre-Jean G. Malé, Benoît Pujol, Christophe Thébaud
- Development of 18 microsatellite markers for the southern purple-spotted gudgeon (Mogurnda adspersa) from the lower Murray-Darling Basin through 454 pyrosequencing
Conservation Genetics Resources DOI:10.1007/s12686-011-9542-0Daniel C. Carvalho, Minami Sasaki, Michael P. Hammer, Luciano B. Beheregaray
- Development of 21 microsatellite markers for the threatened Yarra pygmy perch (Nannoperca obscura) through 454 shot-gun pyrosequencing
Conservation Genetics Resources DOI:10.1007/s12686-011-9413-8Daniel C. Carvalho, Clara J. Rodríguez-Zárate, Michael P. Hammer, Luciano B. Beheregaray
- Development of tetranucleotide microsatellite loci and a non-invasive DNA sampling method for Texas horned lizards (Phrynosoma cornutum)
Conservation Genetics Resources DOI:10.1007/s12686-011-9469-5Dean A. Williams, Cory Leach, Amanda M. Hale, Kristopher B. Karsten, Emmanuela Mujica, Diane Barber, Lee Ann Linam, Nathan Rains
- DNA sampling from eggshell swabbing is widely applicable in wild bird populations as demonstrated in 23 species
Molecular Ecology Resources 11 481-493 DOI:10.1111/j.1755-0998.2010.02953.xDavid Martín‐Gálvez, Juan M Peralta‐Sánchez, Deborah A Dawson, Antonio M Martín‐Platero, Manuel Martínez‐Bueno, Terry Burke, Juan J Soler
- Isolation and characterization of microsatellite loci in Rhododendron ferrugineum (Ericaceae) using pyrosequencing technology
American Journal of Botany 98 e120 -e122 DOI:10.3732/ajb.1000533Chloé E. L. Delmas, Emeline Lhuillier, André Pornon, Nathalie Escaravage
- Isolation and PCR-multiplex genotyping of 18 novel microsatellite markers for the threatened southern pygmy perch (Nannoperca australis)
Conservation Genetics Resources DOI:10.1007/s12686-011-9462-zDaniel C. Carvalho, Michael P. Hammer, Luciano B. Beheregaray
- Isolation of SSR markers for two African tropical tree species, Erythrophleum suaveolens and E. ivorense (Caesalpinioideae)
American Journal of Botany DOI:10.3732/ajb.1000482Jérôme Duminil, Guillaume K. Koffi, Gabriel Debout, Federico Sebastiani, Giovanni G. Vendramin, Myriam Heuertz, Santiago C. González-Martínez, Olivier J. Hardy
- Low level of extrapair parentage in wild zebra finches
Animal Behaviour 79 261-264 DOI:16/j.anbehav.2009.11.031Simon C. Griffith, Clare E. Holleley, Mylene M. Mariette, Sarah R. Pryke, Nina Svedin
- Microsatellite resources for Passeridae species: a predicted microsatellite map of the house sparrow Passer domesticus
Molecular Ecology Resources DOI:10.1111/j.1755-0998.2012.03115.xDeborah A Dawson, Gavin J Horsburgh, Andrew P Krupa, Ian R. K Stewart, Sigrun Skjelseth, Henrik Jensen, Alexander D Ball, Lewis G Spurgin, Maria‐Elena Mannarelli, Shinichi Nakagawa, Julia Schroeder, Carl Vangestel, Gavin N Hinten, Terry Burke
- Molecular phylogeny reveals the existence of two sibling species in the aphid pest Brachycaudus helichrysi (Hemiptera: Aphididae)
Zoologica Scripta DOI:10.1111/j.1463-6409.2012.00531.xJoséphine Piffaretti, Flavie Vanlerberghe‐Masutti, Ashraf Tayeh, Anne‐Laure Clamens, Armelle
Cœur D’acier, Emmanuelle Jousselin
- Multiplexed microsatellite markers for genetic studies of beech
Molecular Ecology Resources DOI:10.1111/j.1755-0998.2011.03094.xS. Lefèvre, S. Wagner, R. J Petit, G. De Lafontaine
- Rise of the machines – recommendations for ecologists when using next generation sequencing for microsatellite development
Molecular Ecology Resources DOI:10.1111/j.1755-0998.2011.03037.xMichael G Gardner, Alison J Fitch, Terry Bertozzi, Andrew J Lowe
- Targeted enrichment of genomic DNA regions for next-generation sequencing
Briefings in Functional Genomics 10 374 -386 DOI:10.1093/bfgp/elr033Florian Mertes, Abdou Elsharawy, Sascha Sauer, Joop M.l.m. Van Helvoort, P.j. Van Der Zaag, Andre Franke, Mats Nilsson, Hans Lehrach, Anthony J. Brookes
- Thirteen microsatellites developed by SSR-enriched pyrosequencing for Solanum rostratum (Solanaceae) and related species
American Journal of Botany 98 e296 -e299 DOI:10.3732/ajb.1100159Mario Vallejo-Marín, Lislie Solis-Montero, Cecile F. E. Bacles, Olivier Lepais
- Twenty-nine microsatellite markers for two Australian freshwater turtles, Elseya albagula and Emydura macquarii krefftii: development from 454-sequence data and utility in related taxa
Conservation Genetics Resources 3 449-456 DOI:10.1007/s12686-010-9377-0Erica Todd, David Blair, Mark Hamann, Dean Jerry
- Two highly validated multiplexes (12‐plex and 8‐plex) for species delimitation and parentage analysis in oaks (Quercus spp.)
Molecular Ecology Resources 11 578-585 DOI:10.1111/j.1755-0998.2011.02983.xE. Guichoux, L. Lagache, S. Wagner, P. Léger, R. J Petit
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